Journal: Cell Cycle

Article Title: Depleted HDAC3 attenuates hyperuricemia-induced renal interstitial fibrosis via miR-19b-3p/SF3B3 axis

doi: 10.1080/15384101.2021.1989899

Figure Lengend Snippet: HN induces RIF. (a). 24 h urine protein, Scr, BUN and UA contents in serum of HN rats; (b). H&E staining analyzed pathological damages of renal tissues of HN rats; (c). Masson staining analyzed the degree of fibrosis and RIF index of HN rats; (d). α-SMA, TGF-β1 and FN contents in renal tissues of HN rats; (e). TUNEL staining detected renal cell apoptosis of HN rats. Data were expressed as mean ± standard deviation. The t-test was used for comparison between two groups. * P < 0.05 compared with the normal group.

Article Snippet: Subsequently, the sections were immersed in 3% H 2 O 2 deionized and blocked with goat serum, followed by treatment with anti-α-smooth muscle actin (α-SMA, 1:200, Invitrogen, CA, USA), anti-transforming growth factor β1 (TGF-β1, 1:200; R&D Systems, MN, USA) and anti-fibronectin (FN, 1:100; Millipore Sigma, MO, USA).

Techniques: Staining, TUNEL Assay, Standard Deviation, Comparison

Journal: Cell Cycle

Article Title: Depleted HDAC3 attenuates hyperuricemia-induced renal interstitial fibrosis via miR-19b-3p/SF3B3 axis

doi: 10.1080/15384101.2021.1989899

Figure Lengend Snippet: Suppressed HDAC3 relieves HN-induced RIF. (a/b). RT-qPCR and Western blot assay detected HDAC3 expression of HN rats; (c/d). RT-qPCR and Western blot assay detected HDAC3 expression of HN rats after down-regulation of HDAC3; (e). 24 h urine protein, Scr, BUN and UA in serum of HN rats after down-regulation of HDAC3; (f). H&E staining analyzed pathological damages of renal tissues of HN rats after down-regulation of HDAC3; (g). Masson staining analyzed the degree of fibrosis and RIF index of HN rats after down-regulation of HDAC3; (h). α-SMA, TGF-β1 and FN contents in renal tissues of HN rats after down-regulation of HDAC3; (i). TUNEL staining detected renal cell apoptosis of HN rats after down-regulation of HDAC3. Data were expressed as mean ± standard deviation. The t-test was used for comparison between two groups. # P < 0.05 compared with the normal group; * P < 0.05 compared with the sh-NC group.

Article Snippet: Subsequently, the sections were immersed in 3% H 2 O 2 deionized and blocked with goat serum, followed by treatment with anti-α-smooth muscle actin (α-SMA, 1:200, Invitrogen, CA, USA), anti-transforming growth factor β1 (TGF-β1, 1:200; R&D Systems, MN, USA) and anti-fibronectin (FN, 1:100; Millipore Sigma, MO, USA).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Staining, TUNEL Assay, Standard Deviation, Comparison

Journal: Cell Cycle

Article Title: Depleted HDAC3 attenuates hyperuricemia-induced renal interstitial fibrosis via miR-19b-3p/SF3B3 axis

doi: 10.1080/15384101.2021.1989899

Figure Lengend Snippet: Elevated miR-19b-3p attenuates HN-induced RIF. (a). RT-qPCR detected miR-19b-3p expression after up-regulation of miR-19b-3p in HN rats; (b). 24 h urine protein, Scr, BUN and UA contents in serum of HN rats after up-regulation of miR-19b-3p; (c). H&E staining analyzed pathological damages of renal tissues of HN rats after up-regulation of miR-19b-3p; (d). Masson staining analyzed the degree of fibrosis and RIF index of HN rats after up-regulation of miR-19b-3p; (e). α-SMA, TGF-β1 and FN contents in renal tissues of HN rats after up-regulation of miR-19b-3p; F. TUNEL staining detected renal cell apoptosis of HNl rats after up-regulation of miR-19b-3p. Data were expressed as mean ± standard deviation. The t-test was used for comparison between two groups. * P < 0.05 compared with the mimic-NC group.

Article Snippet: Subsequently, the sections were immersed in 3% H 2 O 2 deionized and blocked with goat serum, followed by treatment with anti-α-smooth muscle actin (α-SMA, 1:200, Invitrogen, CA, USA), anti-transforming growth factor β1 (TGF-β1, 1:200; R&D Systems, MN, USA) and anti-fibronectin (FN, 1:100; Millipore Sigma, MO, USA).

Techniques: Quantitative RT-PCR, Expressing, Staining, TUNEL Assay, Standard Deviation, Comparison

Journal: Cell Cycle

Article Title: Depleted HDAC3 attenuates hyperuricemia-induced renal interstitial fibrosis via miR-19b-3p/SF3B3 axis

doi: 10.1080/15384101.2021.1989899

Figure Lengend Snippet: miR-19b-3p depletion abrogates down-regulated HDAC3-induced effects on HN-induced RIF. (a/b). RT-qPCR and Western blot assay detected SF3B3 expression after down-regulation of HDAC3 in HN rats; (c/d). RT-qPCR and Western blot assay detected SF3B3 expression after down-regulation of HDAC3 and miR-19b-3p in HN rats; (e). 24 h urine protein, Scr, BUN and UA contents after down-regulation of HDAC3 and miR-19b-3p in HN rats; (f). H&E staining analyzed pathological damages of renal tissues after down-regulation of HDAC3 and miR-19b-3p in HN rats; (g). Masson staining analyzed the degree of fibrosis and RIF index after down-regulation of HDAC3 and miR-19b-3p in HN rats; (h). α-SMA, TGF-β1 and FN contents in renal tissues after down-regulation of HDAC3 and miR-19b-3p in HN rats; (i). TUNEL staining detected renal cell apoptosis after down-regulation of HDAC3 and miR-19b-3p in HN rats. Data were expressed as mean ± standard deviation. The t-test was used for comparison between two groups. & P < 0.05 compared with the sh-NC group; * P < 0.05 compared with the sh-HDAC3 group.

Article Snippet: Subsequently, the sections were immersed in 3% H 2 O 2 deionized and blocked with goat serum, followed by treatment with anti-α-smooth muscle actin (α-SMA, 1:200, Invitrogen, CA, USA), anti-transforming growth factor β1 (TGF-β1, 1:200; R&D Systems, MN, USA) and anti-fibronectin (FN, 1:100; Millipore Sigma, MO, USA).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Staining, TUNEL Assay, Standard Deviation, Comparison

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Hirudin Protects Ang II-Induced Myocardial Fibroblasts Fibrosis by Inhibiting the Extracellular Signal-Regulated Kinase1/2 (ERK1/2) Pathway

doi: 10.12659/MSM.909044

Figure Lengend Snippet: Hirudin regulated fibrosis-related factors in Ang II-induced myocardial fibroblasts. ( A ) The relative mRNA levels of MMP-2, MMP-9, TIMP-2 was evaluated by qRT-PCR. ( B ) The relative protein levels of MMP-2, MMP-9, TIMP-2 was examined by western blot. ( C ) qRT-PCR was performed to determine the mRNA levels of FN, TGF-β1, COL-I and COL-III. ( D ) Western blot was carried out to detect the protein levels of FN, TGF-β1, COL-I and COL-III, and normalized to β-actin expression. Gray value was detected and counted by quality one. * P <0.05, ** P <0.01, *** P <0.001 vs. control. # P <0.05, ## P <0.01, ### P <0.001, vs. Ang II.

Article Snippet: The membranes were incubated with anti-matrix metalloproteinase-2 (MMP-2) (AmyJet Scientific, 12015-01, 1: 2000), anti-MMP-9 (R&D, AF909-SP, 1: 1000), anti-tissue inhibitor of metalloproteinases-2 (TIMP-2) (R&D, AF971, 1: 1200), anti-fibronectin (FN) (Abcam, ab2413, 1: 800), anti-transforming growth factor beta 1 (TGF-β1) (R&D, FAB766G, 1: 700), anti-collagen-I (COL-I) (Abcam, ab90395, 1: 1000), anti-collagen III (COL-III) (Abcam, ab7778, 1: 800), anti-p-ERK1/2 (CST, 8544, 1: 1000), anti-ERK1/2 (CST, 9102, 1: 700), and anti-β-actin (Abcam, ab8226, 1: 2000) at 4°C for 24 hours.

Techniques: Quantitative RT-PCR, Western Blot, Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Protective Mechanism of Nostoc sphaeroides Kütz. Polysaccharide on Liver Fibrosis by HFD-Induced Liver Fat Synthesis and Oxidative Stress

doi: 10.1155/2022/1745244

Figure Lengend Snippet: Effects of NSKP on hepatic TGF-β1 , α-SMA , and FN in high-fat mice. (a) The blot of TGF-β1 by WB. (b) Protein expression of TGF-β1 . (c) Relative quantity of TGF-β1 by RT-PCR. (d) Immunohistochemical staining ( α-SMA , FN ), original magnification: 400x. (e) Quantitative analysis of the average optical density of α-SMA immunohistochemical staining sections. (f) Quantitative analysis of the average optical density of FN immunohistochemical staining sections. (g, h) Relative quantity of α-SMA and FN by RT-PCR. Bars marked with different letters represent statistically significant ( P < 0.05), whereas bars labeled with the same letter indicate no statistically significant difference between the groups ( P > 0.05). Values represent mean ± SEM; n = 10 in each group ( TGF-β1 : transforming growth factor-beta 1; α-SMA : α -smooth muscle actin; FN : fibronectin).

Article Snippet: Antibodies GAPDH (No. G3206-1OD), anti-transforming growth factor-beta 1 ( TGF-β1 , No. GB111876), α-SMA (No. GB13044), and anti-fibronectin ( FN , No. GB11091); Sirius red staining kit; H&E staining kit; and oil red O staining kit were all provided by Wuhan Servicebio Co., Ltd. (Wuhan, China).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Immunohistochemical staining, Staining, Labeling